Journal: American Journal of Physiology - Cell Physiology

Article Title: Whole egg, but not egg white, ingestion induces mTOR colocalization with the lysosome after resistance exercise

doi: 10.1152/ajpcell.00225.2018

Figure Lengend Snippet: Tuberous sclerosis 2 (TSC2) and mechanistic target of rapamycin (mTOR) colocalization with Ras homolog enriched in brain (Rheb) in response to whole eggs or egg whites following resistance exercise. Immunofluorescence quantification of mTOR/TSC2 (red) and Rheb (green) colocalization, displayed as a composite image (merge) and WGA (blue) (n = 10/condition). Yellow/orange regions represent TSC2 and Rheb (A) mTOR and Rheb colocalization (C). Each panel represents one subject from whole eggs and egg whites across the experimental time course. Group data are quantified and reported as TSC2 and Rheb colocalization (B) and mTOR and Rheb colocalization (D). Open circles represent whole eggs and closed circles represent egg whites. All data are presented relative to Fasted. Scale bar represents 100-μm area. Data are presented as means ± SE and were analyzed using two-way repeated-measures ANOVA. *P < 0.01, different from Fasted in same condition. Immunofluorescence image was obtained on a Leica DMI8 confocal microscope of mTOR (red) and Rheb (green) interaction, displayed as a composite image (merge) and WGA (blue) (E). Sections were viewed at ×60 1.30 numerical aperture oil immersion objective to visualize the signal being primarily intracellular and/or within the fiber junctions. Scale bar represents 20-μm area (E).

Article Snippet: The mouse monoclonal anti-mTOR (no. 05-1592) antibody was purchased from Millipore (Toronto, ON, Canada) and mouse monoclonal anti-TSC2 (no. AM1919b-EV) was purchased from FroggaBio (Toronto, ON, Canada).

Techniques: Immunofluorescence, Microscopy